A rapid plate assay for screening l ‐asparaginase producing micro‐organisms

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Rapid and Efficient Dye Based Plate Assay Technique for Screening of L-Asparaginase Producing Fungal Strains

L-Asparaginase enzyme has received great attention owing to its potential as anticancer drug and food processing agent. L-Asparaginase producing microbes are conventionally screened on phenol red plates containing L-asparagine as the sole nitrogen source for its growth. However, the contrast of the zone in phenol red plates is not very distinct. To overcome this problem, an alternate methodolog...

متن کامل

A Rapid Screening Method for Cutinase Producing Microorganisms

Both cutinase and lipase belong to the esterase group of enzymes (EC 3.1.1.X), which are capable of catalyzing the hydrolysis of ester bonds. Cutinase catalyzes the hydrolysis of cutin, an insoluble biopolyester which is the structural component of plant cuticles. As cutinase is an efficient catalyst in watery or organic media, it is potentially appropriate for the detergent, food and cosmetic ...

متن کامل

Rapid plate method for screening hyaluronidase and chondroitin sulfatase-producing microorganisms.

Microbial hyaluronidase and chondroitin sulfatase have been studied because of the possible associations between these enzymes and microbial mechanisms of pathogenicity. One of the most sensitive biological assays for mucopolysaccharide-degrading enzymes is the turbidity reducing unit (TRU) method (3). In most assays for these two enzymes, measurement of activity involves the conjugation of bov...

متن کامل

Microplate Diffusion Assay for Screening of β-Glucanase-Producing Microorganisms

A method is described to screen fungal strains rapidly for overexpression of extracellular β-1,4-endoglucanase in the presence of high levels of sugar compounds. The semi-quantitative assay utilizes microplates in a 96-well format and an azurine dye covalently cross-linked (AZCL) chromogenic substrate. The digestion of AZCL-hydroxyethyl-β-1,4-endoglucanase results in the release of a blue dye d...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 1997

ISSN: 0266-8254,1472-765X

DOI: 10.1046/j.1472-765x.1997.00331.x